abstract：:Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...

journal_title：Molecular reproduction and development

authors： Oliveira RL,Parent A,Cyr DG,Gregory M,Mandato CA,Smith CE,Hermo L

更新日期：2016-06-01 00:00:00

abstract：:Klinefelter syndrome (KS) is the most common genetic cause of human infertility, but the mechanism(s) responsible for its phenotype remain largely unknown. KS is associated with alterations in body composition and with a higher risk of developing metabolic diseases. We therefore hypothesized that KS men seeking fertil...

journal_title：Molecular reproduction and development

authors： Alves MG,Martins AD,Jarak I,Barros A,Silva J,Sousa M,Oliveira PF

更新日期：2016-03-01 00:00:00

abstract：:Myeloid cell leukemia-1 (MCL1), an anti-apoptotic member of the BCL2 family, is expressed abundantly in the testis. Previous characterization revealed that MCL1 is expressed exclusively in the Leydig cells in the mouse testis, yet what it does in these cells remains unknown. We therefore analyzed testosterone biosynth...

journal_title：Molecular reproduction and development

authors： Guang-Yu L,Hai-Yan L,Ji-Hong L,Yun-Cong M,Xue-Lian D,Chun-Yu L,Wen-Yong S

更新日期：2016-03-01 00:00:00

abstract：:Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...

journal_title：Molecular reproduction and development

authors： Ochi H,Aoto S,Tachibana K,Hara M,Chiba K

更新日期：2016-01-01 00:00:00

abstract：:Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryo...

journal_title：Molecular reproduction and development

authors： Krischek C,Janisch S,Naraballobh W,Brunner R,Wimmers K,Wicke M

更新日期：2016-01-01 00:00:00

abstract：:Forward-motility-stimulating factor (FMSF) is a protein, originally purified from bubaline serum, that promotes progressive motility of mature spermatozoa. FMSF binds to sperm surface receptors and activates transmembrane adenylyl cyclase (tmAC), causing a rise in intracellular cyclic AMP level ([cAMP]i) and subsequen...

journal_title：Molecular reproduction and development

authors： Dey S,Roy D,Majumder GC,Mukherjee B,Bhattacharyya D

更新日期：2015-12-01 00:00:00

abstract：:Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells i...

journal_title：Molecular reproduction and development

authors： Wang Y,Zhang JJ,Yang WR,Luo HY,Zhang JH,Wang XZ

更新日期：2015-11-01 00:00:00

abstract：:Development of female gonads in the chicken is asymmetric. This asymmetry affects gene expression, morphology, and germ cell development; consequently only the left ovary develops into a functional organ, whereas the right ovary remains vestigial. In males, on the other hand, both gonads develop into functional testes...

journal_title：Molecular reproduction and development

authors： de Melo Bernardo A,Heeren AM,van Iperen L,Fernandes MG,He N,Anjie S,Noce T,Ramos ES,de Sousa Lopes SM

更新日期：2015-10-01 00:00:00

abstract：:In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...

journal_title：Molecular reproduction and development

authors： Valsangkar DS,Downs SM

更新日期：2015-09-01 00:00:00

abstract：:Maternal immune tolerance to the semi-allogenic fetus is required for successful pregnancy in mammals. Monoclonal nonspecific suppressor factor beta (MNSFB) is an immunosuppressive factor present in uterine epithelial and stromal cells, as well as in macrophages and T cells. Although the functional neutralization of M...

journal_title：Molecular reproduction and development

authors： Gu Y,He Y,Zhang X,Shi Y,Yang Q,Yu L,Sun Z,Zhang H,Wang J,Gao X,Wang J

更新日期：2015-06-01 00:00:00

abstract：:Endometriosis is regarded as a hormone-dependent disease. Current therapeutic approaches to treating this common gynecological disorder mainly depend on surgical and hormonal interventions, but the high rate of disease recurrence as well as the side effects related to such therapies make it difficult for patients to r...

journal_title：Molecular reproduction and development

authors： Che XH,Chen YC,Chen CL,Ye XL,Zhu H

更新日期：2015-06-01 00:00:00

abstract：:The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...

journal_title：Molecular reproduction and development

authors： Lee J,Park JI,Lee GS,Choi JH,Lee ST,Park CK,Kim DY,Hyun SH,Lee E

更新日期：2015-06-01 00:00:00

abstract：:Germ cells require communication with associated somatic cells for normal gametogenesis, as exemplified by an oocyte that interacts with granulosa cells via paracrine factors as well as gap junctions located at sites of contact between these two cell types. The objective of the present study was to define the mechanis...

journal_title：Molecular reproduction and development

authors： McGinnis LK,Kinsey WH

更新日期：2015-02-01 00:00:00

abstract：:The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed to different concentrations of xanthine and xanthine oxidase (X-XO) ...

journal_title：Molecular reproduction and development

authors： Gazo I,Shaliutina-Kolešová A,Dietrich MA,Linhartová P,Shaliutina O,Cosson J

更新日期：2015-01-01 00:00:00

abstract：:In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...

journal_title：Molecular reproduction and development

authors： Gennotte V,Mélard C,D'Cotta H,Baroiller JF,Rougeot C

更新日期：2014-12-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:In the dog, oocyte maturation, fertilization, and early embryo development take place within the oviduct in the presence of increasing circulating progesterone (P4) levels. Expression of the oviduct-specific glycoprotein 1 (OVGP1), known in other species to be estrogen-dependent, was explored by real-time quantitative...

journal_title：Molecular reproduction and development

authors： Saint-Dizier M,Marnier C,Tahir MZ,Grimard B,Thoumire S,Chastant-Maillard S,Reynaud K

更新日期：2014-11-01 00:00:00

abstract：:Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expre...

journal_title：Molecular reproduction and development

authors： Depoix CL,Debiève F,Hubinont C

更新日期：2014-11-01 00:00:00

abstract：:Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...

journal_title：Molecular reproduction and development

authors： Chen X,Yue Y,He Y,Zhu H,Hao H,Zhao X,Qin T,Wang D

更新日期：2014-10-01 00:00:00

abstract：:Spindle positioning is an imperative cellular process that regulates a number of different developmental events throughout embryogenesis. The spindle must be properly positioned in embryos not only for the segregation of chromosomes, but also to segregate developmental determinants into different daughter blastomeres....

journal_title：Molecular reproduction and development

authors： Moorhouse KS,Burgess DR

更新日期：2014-10-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growt...

journal_title：Molecular reproduction and development

authors： Scotti L,Parborell F,Irusta G,De Zuñiga I,Bisioli C,Pettorossi H,Tesone M,Abramovich D

更新日期：2014-08-01 00:00:00

abstract：:cAMP-dependent protein kinase A (PKA) plays important regulatory roles during mouse spermatogenesis. PKA-mediated signaling has been shown to regulate gene expression, chromatin condensation, capacitation, and motility during sperm development and behavior, although how PKA is regulated in spatiotemporal manners durin...

journal_title：Molecular reproduction and development

authors： Xu K,Qi H

更新日期：2014-07-01 00:00:00

abstract：:Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...

journal_title：Molecular reproduction and development

authors： Richani D,Wang X,Zeng HT,Smitz J,Thompson JG,Gilchrist RB

更新日期：2014-05-01 00:00:00

abstract：:Post-acrosomal WW-domain binding protein (PAWP) is a signaling molecule located in the post-acrosomal sheath (PAS) of mammalian spermatozoa. We hypothesized that the proper integration of PAWP in the sperm PAS is reflective of bull-sperm quality and fertility. Cryopreserved semen samples from 298 sires of acceptable, ...

journal_title：Molecular reproduction and development

authors： Kennedy CE,Krieger KB,Sutovsky M,Xu W,Vargovič P,Didion BA,Ellersieck MR,Hennessy ME,Verstegen J,Oko R,Sutovsky P

更新日期：2014-05-01 00:00:00

abstract：:Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune cha...

journal_title：Molecular reproduction and development

authors： Jalali BM,Kitewska A,Wasielak M,Bodek G,Bogacki M

更新日期：2014-03-01 00:00:00

abstract：:Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...

journal_title：Molecular reproduction and development

authors： Zhou J,Huang Y,Li L,Zhu L,Zhang D,Zhang S,Chen Y

更新日期：2013-11-01 00:00:00

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:Fertilization causes mature oocytes or eggs to increase their concentrations of intracellular calcium ions (Ca²⁺) in all animals that have been examined, and such Ca²⁺ elevations, in turn, provide key activating signals that are required for non-parthenogenetic development. Several lines of evidence indicate that the ...

journal_title：Molecular reproduction and development

authors： Kashir J,Deguchi R,Jones C,Coward K,Stricker SA

更新日期：2013-10-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...

journal_title：Molecular reproduction and development

authors： Tian C,Liu J

更新日期：2013-09-01 00:00:00

abstract：:In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine the features and age-associated gene expression profiles of bovine oocytes and embryos as well as to discover possible countermeasures against age-associated events. Comprehensive gene expression assays of germinal ...

journal_title：Molecular reproduction and development

authors： Takeo S,Kawahara-Miki R,Goto H,Cao F,Kimura K,Monji Y,Kuwayama T,Iwata H

更新日期：2013-07-01 00:00:00

abstract：:Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of spe...

journal_title：Molecular reproduction and development

authors： Sim BW,Cha JJ,Song BS,Kim JS,Yoon SB,Choi SA,Jeong KJ,Kim YH,Huh JW,Lee SR,Kim SH,Lee CS,Kim SU,Chang KT

更新日期：2013-03-01 00:00:00

abstract：:Myricetin is a natural flavonoid, particularly enriched in red wines, whose occurrence is widespread among plants. Despite extensive research, the beneficial effects of Myricetin on human health are still controversial. Here, we tested the estrogen-like effect of the phytoestrogen Myricetin on human ejaculated sperm b...

journal_title：Molecular reproduction and development

authors： Aquila S,Santoro M,De Amicis F,Guido C,Bonofiglio D,Lanzino M,Cesario MG,Perrotta I,Sisci D,Morelli C

更新日期：2013-02-01 00:00:00

abstract：:Proteins in the seminal fluid of animals with internal fertilization effect numerous responses in mated females that impact both male and female fertility. Among these proteins is the highly represented class of proteolysis regulators (proteases and their inhibitors). Though proteolysis regulators have now been identi...

journal_title：Molecular reproduction and development

authors： Laflamme BA,Wolfner MF

更新日期：2013-02-01 00:00:00

abstract：:The frameworks (key mechanisms) of the self/non-self-discrimination systems that are found in various organisms have not been actively selected for, but have evolved by genetic drift such that the genetic frequency of random, advantageous mutations has increased within the genomes of these species by natural selection...

journal_title：Molecular reproduction and development

authors： Harada Y,Sawada H

更新日期：2013-01-01 00:00:00

abstract：:In vitro growth of preantral follicles has the potential to produce considerable numbers of competent oocytes for use in medicine, agriculture, and even wildlife conservation. The critical regulatory role of growth factors and hormones in the development of preantral follicles has been established. This study investig...

journal_title：Molecular reproduction and development

authors： Esmaielzadeh F,Hosseini SM,Nasiri Z,Hajian M,Chamani M,Gourabi H,Shahverdi AH,Vosough AD,Nasr-Esfahani MH

更新日期：2013-01-01 00:00:00

abstract：:EPPIN (epididymal protease inhibitor; SPINLW1), an antimicrobial cysteine-rich protein containing both Kunitz and whey acidic protein (WAP)-type four disulfide core protease inhibitor consensus sequences, is a target for male contraception because of its critical role in sperm motility. Here, we characterized EPPIN's ...

journal_title：Molecular reproduction and development

authors： Silva EJ,Patrão MT,Tsuruta JK,O'Rand MG,Avellar MC

更新日期：2012-12-01 00:00:00

abstract：:Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa cDNA (Smvas) from ...

journal_title：Molecular reproduction and development

authors： Lin F,Xu S,Ma D,Xiao Z,Zhao C,Xiao Y,Chi L,Liu Q,Li J

更新日期：2012-11-01 00:00:00

abstract：:Oocyte meiosis is arrested at prophase I by factors secreted from surrounding somatic cells after oocytes acquire meiotic competence at an early antral stage, and meiosis resumes in preovulatory follicles as a result of the luteinizing hormone (LH) surge. Recently, signaling by C-type natriuretic peptide (CNP) through...

journal_title：Molecular reproduction and development

authors： Tsuji T,Kiyosu C,Akiyama K,Kunieda T

更新日期：2012-11-01 00:00:00

abstract：:The use of unrestricted somatic stem cells (USSCs) holds great promise for future clinical applications. Conventionally, mouse embryonic fibroblasts (MEFs) or other animal-based feeder layers are used to support embryonic stem cell (ESC) growth; the use of such feeder cells increases the risk of retroviral and other p...

journal_title：Molecular reproduction and development

authors： Keshel SH,Soleimani M,Tavirani MR,Ebrahimi M,Raeisossadati R,Yasaei H,Afsharzadeh D,Behroz MJ,Atashi A,Amanpour S,Khoshzaban A,Roozafzoon R,Behrouzi GR

更新日期：2012-10-01 00:00:00

abstract：:The present study investigated the effects of triiodothyronine (T3) on pituitary gonadotropin (GTH) subunits, thyroid stimulating hormone (TSH) β subunit, and growth hormone (GH) mRNA levels, as well as gonadal steroid secretion during different stages of reproduction in goldfish. Goldfish pituitary cells cultured wit...

journal_title：Molecular reproduction and development

authors： Allan ER,Habibi HR

更新日期：2012-09-01 00:00:00